Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7
cell line
MCF7
cell type
ER+ breast cancer cell line
genotype
sgNF1-2+sgNR2F2-2
chip antibody
ER (Millipore, #06-935)

Sequenced DNA Library

library_name
GSM6717018
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching, chromatin was prepared using SimpleChIP Enzymatic Chromatin Kit from cell signaling. ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
38047015
Reads aligned (%)
98.7
Duplicates removed (%)
24.6
Number of peaks
898 (qval < 1E-05)

hg19

Number of total reads
38047015
Reads aligned (%)
97.8
Duplicates removed (%)
25.1
Number of peaks
459 (qval < 1E-05)

Base call quality data from DBCLS SRA